![]() However, the methanogenic archaeal community structure was driven by amended methanogenic precursors with a dominance of Methanobacterium in H 2/CO 2-based incubations and Methanosarcina in acetate-based incubations. Temperature was not found to shape the total microbial community structure, that rather exhibited a site-specific variability among the studied lakes. 1.9 eV) compared to incubations spiked with acetate or unamended (c. Temperature dependence of MPRs was significantly higher in incubations spiked with H 2/CO 2 (c. At low temperatures, H 2/CO 2-derived MPRs were considerably lower (down to 160-fold lower) than the acetate-derived MPRs, but the contribution of hydrogenotrophic methanogenesis increased with temperature. Stimulation of methanogenesis by methanogenic precursors as acetate and H 2/CO 2, resulted in an increase of MPRs up to 127-fold and 19-fold, respectively, as well as an enrichment of mcrA-carriers strikingly stronger under acetate amendment. Under unamended conditions, a temperature increase of 5 ☌ doubled MPR while microbial community structure was not affected. We combined in situ CH 4 flux measurements, CH 4 production rates (MPRs), gene abundance quantification and microbial community structure analysis (metabarcoding of the 16S rRNA gene). This study aims to evaluate temperature-induced (from 5 to 20 ☌) changes on microbial community structure and methanogenic pathways in five sub-Antarctic lake sediments from Magallanes strait to Cape Horn, Chile. Freshwater ecosystems are responsible for an important part of the methane (CH 4) emissions which are likely to change with global warming. ![]()
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